User contributions for Admin

1 February 2021

• 19:3819:38, 1 February 2021 diff hist +4,272‎ N Preparation of Silica-Magnetic Beads ‎ Created page with "Silica (SiO₂) beads and silica-coated magnetic beads are a substrate for the attachments of a broad range of biomolecules through polar interactions.  Lipids,..." current
• 19:3819:38, 1 February 2021 diff hist +3,108‎ N Opsonization ‎ Created page with "Opsonization allows phagocytes to efficiently recognize particles, by coating them with opsonins such as IgG isotype antibodies. This page contains the protocols for opsonisin..." current
• 19:3719:37, 1 February 2021 diff hist +691‎ N Water Bath Antibiotic Solution ‎ Created page with "= Reagents Needed = *Copper Sulfate (Cu₂SO₄) *EDTA *Dish soap or other detergent (i.e. Tween/Triton) = Preparation = Fill water bath with tap water (typ..." current
• 19:3719:37, 1 February 2021 diff hist +1,860‎ N Agarose Gels ‎ Created page with "==== Protocol<br> ==== ===== For a 0.7% gel:<br> ===== #Set up gel-casting dock. #Add 0.14 g of agarose to a 250mL Erlenmeyer flask. #Add 400 µL of 50X TAE and 20 ml of d..."
• 19:3719:37, 1 February 2021 diff hist +675‎ N Antibiotic Plates ‎ Created page with "=== Protocol === #Add 20 g of LB agar to a 1 L flash. #Fill flask to the 500 mL line with dH₂O. #Autoclave the flask. Note: have a water bath set to 50°C. #Whe..." current
• 19:3719:37, 1 February 2021 diff hist +988‎ N Antibiotics ‎ Created page with "== Protocol == ==== Ampicillin<br> ==== Preparation of 5 mL of 100 mg/mL: #Add 0.5 g of ampicilin into a 12 mL tube. #Add 2.5 mL of ddH₂O into the tube and mix g..."
• 19:3619:36, 1 February 2021 diff hist +2,253‎ N J774 Cell Transfection ‎ Created page with "= Protocol = ===== Cell Culture<br> ===== #Prepare a 6-well plate by adding 2 ml of J774 Cell media per well. If coverslips are required, add one coverslip/well prior to add..."
• 19:3619:36, 1 February 2021 diff hist +2,285‎ N Raw Cell Transfection ‎ Created page with "= Protocol = ===== Cell Culture<br> ===== #Prepare a 6-well plate by adding 2 ml of Raw Cell media per well. If coverslips are required, add one coverslip/well prior to addi..." current
• 19:2619:26, 1 February 2021 diff hist +1,284‎ N Freezing and Thawing Cells ‎ Created page with "= Freezing Mammalian Cells = #In a T75 flask, grow cells to 80-90% confluency, or 75-80% density for suspended cells. #Collect cells (trypsinize/scrape adherent cells, etc)...." current
• 19:2519:25, 1 February 2021 diff hist +2,796‎ N Primary Macrophage Culture ‎ Created page with "= Isolation and Culture of Mouse Bone Marrow-Derived Macrophages = === Protocol<br> === ===== Materials<br> ===== *Dulbecco's minimal essential medium (DMEM) from Invitroge..."
• 19:2519:25, 1 February 2021 diff hist +1,380‎ N Bacterial Growth Media ‎ Created page with "=== LB Broth<br> === To 200 mL ddH₂O add:<br> *Pre-made: **6.25g Miller LB powder<br> *'''''OR'''''<br> **2.5g Tryptone<br> **2.5g NaCl<br> **1.25g Yeast extract..." current
• 19:2519:25, 1 February 2021 diff hist +2,226‎ N Cell Culture Guidelines ‎ Created page with "= Maintenance of Common Cell Lines = {| width="95%" border="1" cellpadding="1" cellspacing="1" |- | '''Cell Type''' | '''Media''' | '''Maintenance''' | '''Splitting''' | '''N..." current
• 19:2519:25, 1 February 2021 diff hist +4,126‎ N Common buffers ‎ Created page with "= Cell Culture = ===== 1x PBS<br> ===== To 800ml ddH₂O add:<br> *8g NaCl<br> *0.2g KCl<br> *1.44g Na₂HPO₄ (sodium phosphate dibasic)<br>..." current Tag: Visual edit: Switched
• 19:2419:24, 1 February 2021 diff hist −62‎ m Main Page ‎ →‎Lab Operations Tag: Visual edit
• 19:2319:23, 1 February 2021 diff hist +3,139‎ N Setting Up Network Drives ‎ Created page with "The laboratory has a server on which all work-related files must be stored. Before you can access these folders you must get a user ID and password from Dr. Heit.  Please..." current
• 19:2319:23, 1 February 2021 diff hist +2,828‎ N Saving Experimental Files ‎ Created page with "= Logging Computer Files = It is not uncommon for experiments to generate large quantities of computer files containing raw data, analyzed data, statistics, intermediary stag..." current
• 19:2319:23, 1 February 2021 diff hist +7,003‎ N Labbook Guidelines ‎ Created page with "= Preamble<br> = Your laboratory notebook is an important tool that goes well beyond research management and can have important implications for issues ranging from intellect..." current
• 19:2219:22, 1 February 2021 diff hist +1,668‎ N Exit Protocol ‎ Created page with "It is critical that members leaving the lab properly archive all materials so that they can be found and used by others.  This is critical to the success of both yourself..."
• 19:2219:22, 1 February 2021 diff hist +6,837‎ N Entrance Protocol ‎ Created page with "Beginning in a new lab can be an overwhelming experience.  This guide is intended to aid new members in starting up as quickly as possible. = Accounts & Certificatio..."
• 19:2119:21, 1 February 2021 diff hist +5,494‎ N Reducing Photobleaching ‎ Created page with "Photobleaching is a common issue with both fixed and live samples. Proper sample preparation, and the addition of photoprotectants, can dramatically reduce the degree of photo..." current Tag: Visual edit: Switched
• 19:1819:18, 1 February 2021 diff hist +673‎ N Create new ‎ Created page with "= How to Create a New Page = #On the main page, in the index section, add the title of your new page. #Highlight the new title, then click the link icon on the..." current
• 19:1719:17, 1 February 2021 diff hist +2,581‎ N Editing ‎ Created page with "= Instructions for Creating or Editing Pages = To make the pages consistent, and protocols easy to follow, please follow these guidelines: #Structure the protocol in the ord..." current Tag: Visual edit: Switched
• 18:5618:56, 1 February 2021 diff hist +10,121‎ Main Page ‎No edit summary
• 18:4218:42, 1 February 2021 diff hist 0‎ N File:Logo.png ‎No edit summary current