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• 18:52, 17 January 2025 Akipp4 talk contribs created page Restriction Digest (Created page with " Protocol 1. Check the NEB (or other supplier) website to confirm enzyme compatibility 2. In a PCR tube add the reaction components according to the table listed below 3. Incubate at 37°C for 30 min if time-saver qualified, 60 minutes if not Example Reaction Component Volume ddH2O Up to 50 µL 10X rCutSmart Buffer 5 µL DNA x (1 µg) Enzyme 1 1 µL Enzyme 2 1 µL Notes - NEB High-Fidelity (HF) enzymes are ordered whenever possible, they all use rCutSmart reactio...")
• 19:02, 29 July 2024 Admin talk contribs created page THP1 Culture and Differentiation (Created page with "==== Maintenance ==== THP1 cells ([https://www.atcc.org/products/tib-202?matchtype=&network=x&device=c&adposition=&keyword=&gad_source=1&gclid=Cj0KCQjw-5y1BhC-ARIsAAM_oKlZTKs5zplX22cd1pVZB7E0-Cq7fDL5pcruuijrK0PqV9a-5LG5l24aAoEqEALw_wcB ATCC TIB-202]) should be maintained in RPMI + 10% FBS, with cells split 1:5 into fresh medium when the cell density reaches 1 x 10⁶ cells/mL. Do not allow cell density to exceed 1.5 x 10⁶ cells/mL. '''Classical Mac...") Tag: Visual edit
• 15:12, 10 July 2024 Admin talk contribs created page Frustrated Phagocytosis (Created page with "==== Before the Experiment ==== # Prepare substrates and place them into the wells of a 12-well plate. These can be prepared by coating slides with a ligand of interest, or by microprinting. # Transfect cells if required, as per usual protocols. # Split macrophages into wells without coverslips, 1 well/frustrated phagocytosis assay (~250,000 cells/well). ==== Experiment: Fixed-Cell ==== # Pre-warm the substrates and centrifuges to 37C. # I...") Tag: Visual edit
• 16:43, 19 June 2024 Admin talk contribs created page Micropatterning Proteins (Created page with "This protocol is based on IgG micropatterning methods as published by Freeman ''et al''. <ref>Freeman et al., (2016) “Integrins Form an Expanding Diffusional Barrier That Coordinates Phagocytosis.” Cell. V164#1, pp 128-40. http://linkinghub.elsevier.com/retrieve/pii/S0092867415015676</ref>It may need to be optimized for other proteins. ==== Preparation of the Microprinting Stamp. ==== This is optimized for a PDMS based stamp and may have to be modified for stamps m...") Tag: Visual edit
• 15:36, 3 March 2022 Admin talk contribs created page File:MagnetTool.png
• 15:36, 3 March 2022 Admin talk contribs uploaded File:MagnetTool.png
• 15:35, 3 March 2022 Admin talk contribs created page File:50mm BasePlate.png
• 15:35, 3 March 2022 Admin talk contribs uploaded File:50mm BasePlate.png
• 15:35, 3 March 2022 Admin talk contribs created page File:18mm BasePlate.png
• 15:35, 3 March 2022 Admin talk contribs uploaded File:18mm BasePlate.png
• 18:01, 2 March 2022 Admin talk contribs created page File:ResinPrint.png
• 18:01, 2 March 2022 Admin talk contribs uploaded File:ResinPrint.png
• 17:54, 2 March 2022 Admin talk contribs created page File:Resin.png
• 17:54, 2 March 2022 Admin talk contribs uploaded File:Resin.png
• 17:46, 2 March 2022 Admin talk contribs created page File:Assembly.png
• 17:46, 2 March 2022 Admin talk contribs uploaded File:Assembly.png
• 17:36, 2 March 2022 Admin talk contribs created page File:18mm Lid Clear.png
• 17:36, 2 March 2022 Admin talk contribs uploaded File:18mm Lid Clear.png
• 17:36, 2 March 2022 Admin talk contribs created page File:18mm Lid Solid.png
• 17:36, 2 March 2022 Admin talk contribs uploaded File:18mm Lid Solid.png
• 17:36, 2 March 2022 Admin talk contribs created page File:18mm Align.png
• 17:36, 2 March 2022 Admin talk contribs uploaded File:18mm Align.png
• 17:35, 2 March 2022 Admin talk contribs created page File:50mm Lid Clear.png
• 17:35, 2 March 2022 Admin talk contribs uploaded File:50mm Lid Clear.png
• 17:35, 2 March 2022 Admin talk contribs created page File:50mm Lid Solid.png
• 17:35, 2 March 2022 Admin talk contribs uploaded File:50mm Lid Solid.png
• 17:35, 2 March 2022 Admin talk contribs created page File:50mm Align.png
• 17:35, 2 March 2022 Admin talk contribs uploaded File:50mm Align.png
• 17:03, 2 March 2022 Admin talk contribs created page 3D Printed Microscopy Chambers (Created page with "Customized imaging chambers can be 3D printed using FDM or SLA printing technology, and attached to a #1.5 thickness coverglass to allow for high-resolution imaging. When prepared properly, these chambers show no cytotoxicity or other signs of altered cell behaviour, even in cells cultured for multiple days. These are the chamber designs used in the [https://www.phagocytes.ca Heit Lab], and are based on our publication in Biochemistry and Cell Biology<ref name=":0">Teppe...") Tag: Visual edit
• 17:03, 2 March 2022 Admin talk contribs created page File:18mm 4well.png
• 17:03, 2 March 2022 Admin talk contribs uploaded File:18mm 4well.png
• 17:02, 2 March 2022 Admin talk contribs created page File:18mm 2well.png
• 17:02, 2 March 2022 Admin talk contribs uploaded File:18mm 2well.png
• 17:02, 2 March 2022 Admin talk contribs created page File:18mm 1well.png
• 17:02, 2 March 2022 Admin talk contribs uploaded File:18mm 1well.png
• 17:01, 2 March 2022 Admin talk contribs created page File:50mm 15well.png
• 17:01, 2 March 2022 Admin talk contribs uploaded File:50mm 15well.png
• 17:01, 2 March 2022 Admin talk contribs created page File:50mm 12well.png
• 17:01, 2 March 2022 Admin talk contribs uploaded File:50mm 12well.png
• 17:00, 2 March 2022 Admin talk contribs created page File:50mm 10well.png
• 17:00, 2 March 2022 Admin talk contribs uploaded File:50mm 10well.png
• 17:00, 2 March 2022 Admin talk contribs created page File:50mm 9well.png
• 17:00, 2 March 2022 Admin talk contribs uploaded File:50mm 9well.png
• 17:00, 2 March 2022 Admin talk contribs created page File:50mm 8well.png
• 17:00, 2 March 2022 Admin talk contribs uploaded File:50mm 8well.png
• 16:59, 2 March 2022 Admin talk contribs created page File:50mm 6well Long.png
• 16:59, 2 March 2022 Admin talk contribs uploaded File:50mm 6well Long.png
• 16:59, 2 March 2022 Admin talk contribs created page File:50mm 6well.png
• 16:59, 2 March 2022 Admin talk contribs uploaded File:50mm 6well.png
• 16:58, 2 March 2022 Admin talk contribs created page File:50mm 4well Long.png